Figure 1

Increased expression of ADAM10 in higher-stage osteosarcoma tissue. The panels show representative images of the cytoplasmic ADAM10 domain (red dots) merged with 4′,6-diamidino-2-phenylindole (DAPI)-stained nuclei (blue) taken from osteosarcoma tissues pathologically staged from IA to IIB. Specific ADAM10 staining was controlled by using isotype-specific control antibody (control immunoglobulin G (IgG)). The lower-right graph shows statistical analysis of the number of ADAM10⁺ tumor cells in osteosarcoma tissues at different stages. ADAM10: A disintegrin and metalloproteinase 10. Scale bar, 20 μm. *P < 0.05.