Figure 1

Characterisation of induced pluripotent stem cells and the derived hepatocyte-like cells and endodermal progenitors. A: The morphology of an E-iPSCs colony (left panel) and immunofluorescence-based detection of the expression of the pluripotency-specific protein OCT4 (co-staining of nuclei with DAPI- right panel). B: Morphology of HLCs derived from E-iPSCs (left panel) and the expression of the mature liver specific protein ALBUMIN (co-staining of nuclei with DAPI- right panel). C: Morphology of a representative endodermal progenitor colony derived from E-iPSCs and cultured on mouse embryonic feeder cells (left panel). Expression of the self-renewal specific protein LGR5 (middle panel), overlay of LGR5 expressing cells and DAPI stained cell nuclei (right panel). Scale bars 200μm.