Figure 5

Immunofluorescence localization of STELLA and DAZL in human umbilical cord mesenchymal stem cell (HUMSC)-derived germ cell-like cells. Staining for STELLA (red) (A, B, E) and DAZL (red) (C, D, F) in differentiated cells (A, C, E, F) on day 14 after co-culture and HUMSCs cultured alone (B, D) were performed and examined by immunofluorescence microscopy (A-D) and confocal microscopy (E, F). Nuclei were counterstained with DAPI (blue). Arrowheads indicate nuclear staining, and arrows indicate cytoplasmic staining. (Magnification 200×).