Fig. 5
From: Beneficial reward-to-risk action of glucosamine during pathogenesis of osteoarthritis
Effect of short time exposure vs. long time exposure of glucosamine. a Normal human chondrocytes were treated with 50 mM glucosamine for 2 or 24 h and the autophagosome formation was analyzed by introducing LC3-GFP constructs. b Normal human chondrocytes were left untreated or were treated with 10, 30, 50, or 100 mM glucosamine for 2 or 24 h. Cell morphology was detected by phase-contrast microscopy and peroxidation was analyzed by BODIPY 665/676 staining (upper panel). The RNA levels of ACBD5 and ACOX1 were analyzed by qRT-PCR (lower panel). Results shown are representative of at least four independent experiments. c RNA levels of COMP and ACAN were analyzed by qRT-PCR. Results shown are representative of at least four independent experiments