Fig. 2

Amplifications of CYP1A1 m2 and the RFLP products of the polymorphic forms: a PCR amplification for CYP1A1 m2 (204 bp). b CYP1A1 m2 polymorphism detected by RFLP. The 204-bp PCR product was digested with BsrDI enzyme. The cleavage site is lost in case of variants to give a single amplicon, whereas the wild-type allele generates 149- and 55-bp fragments. Lane 5 represents homozygous minor (GG); Lane 7 represents the heterozygote (AG); Lanes 2–4, 6 and 8–13 represent homozygous major (AA)