Table 1 Antibodies used for flow cytometry
From: The inflammatory phase of fracture healing is influenced by oestrogen status in mice
Antibody | Label | Product | Company | Dilution |
|---|---|---|---|---|
Il-6 (rabbit anti-mouse) | – | bs-0782R | Bioss | 1:250 |
Mdk (goat anti-mouse) | – | sc-1398 | SantaCruz | 1:100 |
CCL2 (rabbit anti-mouse) | – | bs-1955R | Bioss | 1:150 |
CXCL1 (rabbit anti-mouse) | – | ab86436 | Abcam | 1:200 |
Ly6G (rat anti-mouse) | – | 127603 | BioLegend | 1:300 |
CD45/B220 (rat anti-mouse) | – | RA3-6B2 | BioLegend | 1:100 |
CD8 (rabbit anti-mouse) | – | bs-0648R | Bioss | 1:100 |
F4/80 (rat anti-mouse) | – | ab6640 | AbD Serotec | 1:500 |
Streptavidin | HRP | ZUC012 | Zytomed Sytems | 1:100 |
IgG (donkey anti-goat) | Biotin | sc-3854 | SantaCruz | 1:100 |
IgG (goat anti-rabbit) | Biotin | B2770 | Life Technologies | 1:100 |
IgG (goat anti-rat) | Biotin | 31830 | Invitrogen | 1:100 |