Table 2 Antibodies used for immunohistochemistry
From: The inflammatory phase of fracture healing is influenced by oestrogen status in mice
Antibody | Label | Product | Company | Dilution |
|---|---|---|---|---|
CD3e (Armenian hamster anti-mouse) | PE-Cyanine 7 | 25-0031 | Affymetrics eBioscience | 1:100 |
CD4 (rat anti-mouse) | APC-eFlour® 780 | 47-0041 | Affymetrics eBioscience | 1:200 |
CD8a (rat anti-mouse) | APC | 17-0081 | Affymetrics eBioscience | 1:800 |
CD11b (rat anti-mouse) | Alexa Flour® 700 | 56-0112 | Affymetrics eBioscience | 1:400 |
CD19b (rat anti-mouse) | PE | 12-0193 | Affymetrics eBioscience | 1:400 |
F4/80 (rat anti-mouse) | FITC | 11-4701 | Affymetrics eBioscience | 1:50 |
Ly-6G (rat anti-mouse) | V450 | 560603 | BD Bioscience | 1:400 |
IgG Isotype (Armenian hamster) | PE-Cyanine 7 | 25-4888 | Affymetrics eBioscience | 1:100 |
IgG2b K Isotype (rat) | APC-eFlour® 780 | 47-4031 | Affymetrics eBioscience | 1:200 |
IgG2a K Isotype (rat) | APC | 17-4321 | Affymetrics eBioscience | 1:800 |
IgG2b K Isotype (rat) | Alexa Flour® 700 | 56-4031 | Affymetrics eBioscience | 1:400 |
IgG2a K Isotype (rat) | PE | 12-4321 | Affymetrics eBioscience | 1:400 |
IgG2a K Isotype (rat) | FITC | 11-4321 | Affymetrics eBioscience | 1:50 |
IgG2a K Isotype (rat) | V450 | 560377 | BD Bioscience | 1:400 |