Fig. 4

Toxicity testing of the rinsing solutions with C2C12-cells. a Alamar blue testing of relative cell survival. b LDH testing to detect cell death. The testing shows that no flowout of the washing steps contains SDS in concentrations inducing toxicity to the indicator cells. DMEM: cells cultivated in 100% DMEM; PBS: cells cultivated in 100% PBS; DMEM + PBS: cells cultivated in 1:1 mixture of DMEM and PBS; DMEM + SDS and DMEM + Triton-X: 1:1 mixture of DMEM and SDS, respectively, Triton X-100 in given concentration in PBS; DMEM + washing: 1:1 mixture of DMEM and collected flowout from the certain washing step