Fig. 6

Histology at 5 days after seeding primary human osteoblasts into the decellularized kidneys. Decellularization was performed with 0.66% SDS for 60 min. a Overview magnification 10×. b Detailed section magnification 32×. Histology reveals no signs for matrix toxicity. After 5 days of culture, cells are growing in large areas of the matrix. Cell shape appears normal without signs of apoptosis or necrosis. Representative pictures. Black bar indicate 100 µm, Red bar indicate 30 µm. n = 3