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Table 1 Steps of the decellularization procedure

From: Optimized protocol for whole organ decellularization

Step Perfusion solution Volume (ml) Perfusion time
1 PBS 200 10 min
2 SDS (concentration variable, compare Table 2) 300 Variable (compare Table 2)
3 PBS (1st washing step) 200 60 min
4 PBS (2nd washing step) 200 60 min
5 PBS (3rd washing step) 200 60 min
6 PBS (4th washing step) 200 60 min
7 PBS (5th washing step) 200 60 min