Optimized protocol for whole organ decellularization

Table 1 Steps of the decellularization procedure

Step	Perfusion solution	Volume (ml)	Perfusion time
1	PBS	200	10 min
2	SDS (concentration variable, compare Table 2)	300	Variable (compare Table 2)
3	PBS (1st washing step)	200	60 min
4	PBS (2nd washing step)	200	60 min
5	PBS (3rd washing step)	200	60 min
6	PBS (4th washing step)	200	60 min
7	PBS (5th washing step)	200	60 min

ISSN: 2047-783X