Optimized protocol for whole organ decellularization

Table 2 Overview over the decellularization efficacy of the different protocols

Perfusion SDS concentration (%)	30 min	60 min	120 min
0.25	✗	✗	✓
0.50	✗	✗	✓
0.66	✓	✓	✓
1.00	✗	✓	✓

Three rat kidneys were decellularized for each condition (Perfusion time/SDS concentration). Success of decellularization was investigated macroscopically and with histology (HE-Staining). ✓: complete decellularization of all three kidneys; x: Detectable cell residues in at least one kidney. Perfusion for at least 60 min with at least 0.66% SDS results in reliable decellularization. n = 3

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