Fig. 5

Effect of NAC to suppress MeHg-induced MIP-2 mRNA expression. RAW264.7 cells were treated with 1 or 20 mM NAC at 1 h before (pre-treatment), with (co-treatment), or 1 h/3 h after 2 µM MeHg (post-treatment) for 3 h of incubation. mRNA expression levels of MIP-2 were analyzed by real-time PCR values which were normalized to the expression of β-actin and it represents three independent experiments. MIP-2 mRNA expression induced by MeHg treatment alone is compared with control, P = 0.0369 (labelled as ^#), MeHg + NAC treatment (1, 20 mM) effects are compared with the MeHg treatment alone and the significant values (P = 0.0495) were shown in the figure (labelled as *)