Fig. 2

Histological and NLO microscopy images of capsule with Baker grade 3 (example 2). a An overview of a capsule biopsy with Baker grade 3 stained with hematoxylin and eosin (H&E) with representative magnified images stained with H&E and Masson Goldner’s Trichrome (MT). MT Green stained for connective tissue and red for muscle fibers. b Non-linear optical (NLO) microscopy images with secondary- and third harmonic generation (SHG, THG) and two-photon excitation fluorescence (TPEF) images. SHG Collagen I & II (COLI and COLII), THG tissue morphology and TPEF fibrillar structure represent elastin. c Tissues immunohistochemically stained with COLI, lysyl oxidase (LOX) and alpha-smooth muscle actin (α-SMA) with the respective magnified images. Mouse and rabbit isotype were included as negative controls