Fig. 8
Ingenol mebutate interferes with human epithelial keratinocytes (HEK) migration in a dose-dependent manner. HEK migration was studied in a scratch-assay stimulated by different concentrations of ingenol mebutate (IM). The picture pairs (a–b; c–d; e–f; g–h; i–j; k–l) show the migration of the HEK for the time-points t = 0 h and t = 22 h for different concentrations of IM (colors match concentrations in graph m). m Shows the relative scratch width of three independent assays over 40 h for different concentrations. Interestingly, IM applied in a concentration of 10−7 significantly inhibited cell migration, whereas all other concentrations did not influence keratinocyte migration. Significances were calculated by an unpaired Student’s t test (*p < 0.05, **p < 0.01 and ***p < 0.001); M molar