Fig. 2

Result of ALT release in hepatic effluent. SHBD group: Animals underwent a period of 30 min of hypovolemic shock. 24 h later livers were explanted and prior to reperfusion underwent either 18 h of CS (SHBD, N = 6) or 17 h of CS with 60 min VSOP (SHBD + VSOP, N = 6). NHBD group: Prior to liver explantation, cardiac arrest was induced by phrenotomy for 30 min. Reperfusion was performed either after 18 h of CS (NHBD; N = 6) or 17 h of CS with 60 min VSOP (NHBD + VSOP, N = 6). NHBD served as a negative control group. HBD group: Liver explantation was performed under heart-beating conditions. Reperfusion was performed either after 18 h of CS (HBD; N = 6) or 17 h of CS with 60 min VSOP (HBD + VSOP, N = 6). HBD served as positive control group. During reperfusion, hepatic effluent was collected at 5, 15, 30, 45 and 60 min and enzyme release measured. a Results of all six groups ar shown. b For a better overview, only the results of HBD and SHBD groups are depicted. Major significances are shown; *p < 0.05; **p < 0.005; ***p < 0.001; ****p < 0.0001