Fig. 4

Measurement of bile production during reperfusion. SHBD group: Animals underwent a period of 30 min of hypovolemic shock. 24 h later livers were explanted and prior to reperfusion underwent either 18 h of CS (SHBD, N = 6) or 17 h of CS with 60 min VSOP (SHBD + VSOP, N = 6). NHBD group: Prior to liver explantation, cardiac arrest was induced by phrenotomy for 30 min. Reperfusion was performed either after 18 h of CS (NHBD; N = 6) or 17 h of CS with 60 min VSOP (NHBD + VSOP, N = 6). NHBD served as negative control group. HBD group: Liver explantation was performed under heart-beating conditions. Reperfusion was performed either after 18 h of CS (HBD; N = 6) or 17 h of CS with 60 min VSOP (HBD + VSOP, N = 6). HBD served as positive control group. In all animals, the common bile duct was cannulated and bile production was measured throughout reperfusion. The total amount of bile after 60 min of reperfusion was used for calculation. Major significances are shown; *p < 0.05; **p < 0.005; ***p < 0.001; ****p < 0.0001