Fig. 6
Hepatic oxygen uptake. SHBD group: Animals underwent a period of 30 min of hypovolemic shock. 24 h later livers were explanted and prior to reperfusion underwent either 18 h of CS (SHBD, N = 6) or 17 h of CS with 60 min VSOP (SHBD + VSOP, N = 6). NHBD group: Prior to liver explantation cardiac arrest was induced by phrenotomy for 30 min. Reperfusion was performed either after 18 h of CS (NHBD; N = 6) or 17 h of CS with 60 min VSOP (NHBD + VSOP, N = 6). NHBD served as negative control group. HBD group: Liver explantation was performed under heart-beating conditions. Reperfusion was performed either after 18 h of CS (HBD; N = 6) or 17 h of CS with 60 min VSOP (HBD + VSOP, N = 6). HBD served as positive control group. Perfusat samples were taken at the portal inflow and from the venous effluent and oxygen content was measured immediately in a pH–blood gas analyser. Oxygen uptake was calculated from the differences between portal and venous sites and expressed according to transhepatic flow and liver mass. a Time point 5 min after reperfusion: HBD vs. NHBD. b Time point 60 min after reperfusion: HBD vs. NHBD. c Time point 5 min after reperfusion: HBD vs. SHBD. d Time point 60 min after reperfusion: HBD vs. SHBD. Major significances are shown; *p < 0.05; **p < 0.005; ***p < 0.001; ****p < 0.0001