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Fig. 4 | European Journal of Medical Research

Fig. 4

From: Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1

Fig. 4

Myricetin alleviated the injury and inflammatory factors expression of LPS-stimulated cardiomyocytes H9c2 cells. a H9c2 cells viability was detected with the CKK-8 method. H9c2 cells were pre-incubated with myricetin at the indicated concentration (0, 10, 30, and 50 μM) for 12 h and then stimulated with 10 μg/mL LPS or not for 6 h. b Flow cytometry was applied to observe the apoptotic cells after staining with Annexin V-FITC/PI. c Protein involved in apoptosis was detected by Western blot. d Inflammatory factors were detected with qRT-PCR at mRNA level. e Protein concentration of inflammatory factors was analyzed with ELISA. f Inflammatory factors were detected with Western blot at protein level. H9c2 cells were pre-incubated with myricetin (50 μM) for 12 h and then stimulated with or without 10 μg/mL LPS for 6 h. *P < 0.05, **P < 0.01 or ***P < 0.001. Data were presented as mean ± standard deviation (SD) of at least three independent experiments. LPS lipopolysaccharide, MCP-1 monocyte chemo-attractant protein-1, IL-6 interleukin-6, CCK-8 cell counting kit-8, Annexin V-FITC/PI Annexin V-fluorescein isothiocyanate/propidium iodide, qRT-PCR quantitative real-time PCR, ELISA enzyme-linked immune sorbent assay

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