Fig. 7

Myricetin mitigated LPS-induced inflammatory injury of H9c2 cells via inactivating NF-κB signaling pathway. a After transfection with sh-NC and sh-MALAT1, MALAT1 was determined with qRT-PCR. b H9c2 cell viability was detected with CCK-8 assay. c The apoptotic cells were observed with flow cytometry after staining with Annexin V-FITC/PI. d Western blot assay was conducted to detect protein expression associated with apoptosis. e The expression of MCP-1 and IL-6 at mRNA level was determined with qRT-PCR. f ELISA was used to assess the concentration of MCP-1 and IL-6. g Western blot assay was performed to detect the expression of MCP-1 and IL-6. h The expression of p-/t-p65 and p-/t-IκBα was determined with Western blot assay. After transfection with sh-MALAT1, sh-NC, or not, H9c2 cells were pre-incubated with 50 μM myricetin for 12 h and then stimulated with or without 10 μg/mL LPS 6 h. *P < 0.05 or **P < 0.01. Data were presented as mean ± standard deviation (SD) of at least three independent experiments. LPS lipopolysaccharide, MCP-1 monocyte chemo-attractant protein-1, IL-6 interleukin-6, CCK-8 cell counting kit-8, Annexin V-FITC/PI Annexin V-fluorescein isothiocyanate/propidium iodide, qRT-PCR quantitative real-time PCR, ELISA enzyme-linked immune sorbent assay, sh short hairpin, NC negative control, MALAT1 metastasis-associated lung adenocarcinoma transcript 1