Fig. 3
From: Dok-3 deficient mice display different immune clustering and Tim-3 expression
Macrophages and MDSCs proportion and the Tim-3 expression on these cells in two group mice. Spleen immune cells were isolated from 129 mice (n = 6) and Dok-3−/− mice (n = 6). a Flow cytometry (FCM) analysis of Tim-3 expression on macrophages marked by CD3−CD11b+ (left), and the statistical graph was shown (right), macrophages (129, 12.33 ± 2.131; Dok-3−/−, 31.67 ± 3.336) and Tim-3 expression (129, 28.61 ± 4.120; Dok-3−/−, 16.18 ± 1.874). b Flow cytometry (FCM) analysis of Tim-3 expression on MDSCs marked by CD3−CD11b+ (left), and the statistical graph was shown (right), MDSCs (129, 9.72 ± 1.886; Dok-3−/−, 23.73 ± 3.150) and Tim-3 expression (129, 19.58 ± 1.661; Dok-3−/−, 12.58 ± 1.315). Both the macrophages and MDSCs’ gated strategy are shown in Additional file 1: Figure S1. *p < 0.05, **p < 0.01, ***p < 0.001