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Table 3 Enzyme kinetic parameters of nitrocefin hydrolysis by the NDM-1 enzyme in the absence and presence of streptomycin and amikacin antibiotics in single and in combination as well as in the absence and presence of streptomycin and ciprofloxacin antibiotics in single and in combination

From: A mechanistic approach to prove the efficacy of combination therapy against New Delhi metallo-β-lactamases producing bacterial strain: a molecular and biochemical approach

 

Km (µM)

Kcat (s−1)

Kcat/Km (µM−1s−1)

NDM-1

457.25

12,500

27.337

NDM-1 + streptomycin (1:1)

512.125

25,000

48.816

NDM-1 +amikacin (1:1)

509.66

16,666

32.699

NDM-1 +streptomycin + amikacin

(1:1:1)

498.333

6896.4

13.838

NDM-1 +ciprofloxacin (1:1)

337.115

15,384.6

45.636

NDM-1 + streptomycin + ciprofloxacin (1:1:1)

346.632

6666

19.230

  1. Data are reported as an average of ± standard error from three independent experiments