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Table 3 Enzyme kinetic parameters of nitrocefin hydrolysis by the NDM-1 enzyme in the absence and presence of streptomycin and amikacin antibiotics in single and in combination as well as in the absence and presence of streptomycin and ciprofloxacin antibiotics in single and in combination

From: A mechanistic approach to prove the efficacy of combination therapy against New Delhi metallo-β-lactamases producing bacterial strain: a molecular and biochemical approach

 Km (µM)Kcat (s−1)Kcat/Km (µM−1s−1)
NDM-1457.2512,50027.337
NDM-1 + streptomycin (1:1)512.12525,00048.816
NDM-1 +amikacin (1:1)509.6616,66632.699
NDM-1 +streptomycin + amikacin
(1:1:1)
498.3336896.413.838
NDM-1 +ciprofloxacin (1:1)337.11515,384.645.636
NDM-1 + streptomycin + ciprofloxacin (1:1:1)346.632666619.230
  1. Data are reported as an average of ± standard error from three independent experiments