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Table 1 Overview of the revised articles

From: Extended liver resection in mice: state of the art and pitfalls—a systematic review

Year Work-group Publication Resection method Duration of the intervention (min) Anesthesia Mouse Extent of the liver resection (%) Mortality Observation time
Strain Age (w) Weight (g)
1953 Yokoyoma et al Regeneration of mouse liver after partial hepatectomy Suture ligature of left and medial lobe (Brues, Drury, and Brues) Ether Strain A white 13 24–27,5 65 3/4, 1, 2, 3, 4, 5, 6, 7, 8, 10, 14, 21, 28, 38–45 days
2, 4, 6 m
2004 Nikfarjam et al A model of partial hepatectomy in mice Hemostatic clip Ketamine/ Xylazine + Carprofen CBA 8–10 20–25 70 2% 21 days
2004 Garbow et al MRI measurement of liver regeneration in mice following partial hepatectomy Suture ligature of left lateral lobe
Suture ligature of right median lobe
Suture ligature of left median lobe
20–25 Isoflurane 67
2006 Inderbitzin et al Regenerative capacity of individual liver lobes in the microsurgical mouse model BALB/c 6–8 20–25 75 24, 48 h
2008 Mitchell et al A reproducible and well–tolerated method for 2/3 partial hepatectomy in mice Two Suture ligature Isoflurane + Buprenorphine 8–14 67
2008 Boyce et al A detailed methodology of partial hepatectomy in the mouse Suture ligature Isoflurane + Buprenorphine 70 4%
2008 Martins et al Hepatic lobectomy and segmentectomy models using microsurgical techniques Microscope assisted
Catheter-guided Ligature
15 70
2009 Sorg et al Consequences of surgical stress on the kinetics of skin wound healing: partial hepatectomy delays and functionally alters dermal repair Suture ligature of right upper left upper and left lower lobe Ketamine/Xylazine SKH1-hr 8–12 35–45 70 0% 10 days
2010 Zhang et al The benefits of ligating the lobar portal triads before partial hepatectomy in the mouse Ligature of the lobar portal triads + Suture ligature of left lateral lobe and median lobes 15–20 Isoflurane + Bupivacaine C57BL/6 8–12 20–25 70 1% 7d
2011 Hori et al Simple and sure methodology for massive hepatectomy in the mouse Suture ligature
Hemostatic Clip
Electrocautery scalpels
Suture: 30
Clip: 10
Isoflurane + Buprenorphine C57BL/6 10–20 25  > 70 50% 180 h
2012 Bonninghof et al Effect of different liver resection methods on liver damage and regeneration factors VEGF and FGF-2 in mice Suture Ligature Ketamine/ Xylazine + Buprenorphine BALB/c 8–10 20–25 50 and 70 2% 10 min
3, 24, 48, 120, 240 h
2012 Hori et al Simple and reproducible hepatectomy in the mouse using the clip technique Suture ligature
Hemostatic clip
Suture: 30
Clip: 10
Isoflurane + Buprenorphine C57BL/6 10–20 25 75 and 80 75% resection: 40%
80% resection: 90%
180 h
2014 Xie et al Monitoring of systemic and hepatic hemodynamic parameters in mice
2015 Nevzorova et al Partial hepatectomy in mice Suture ligature of left lateral lobe and left portion of the median lobe Ketamine/xylazine and isoflurane 8–14    5% 0.5, 2, 4, 6 h
2016 Forbes et al Liver regeneration—mechanisms and models to clinical application
2016 Orsini et al MRI as Primary End Point for Pharmacologic Experiments of Liver Regeneration in a Murine Model of Partial Hepatectomy Suture ligature of left lobe and median lobe 30 Isoflurane + Buprenorphine C57BL/6 J 12–14   4 days
2016 Xu et al A reliable scoring system after major liver resection in mice Suture ligature of left lateral lobe and median lobe Isoflurane + Buprenorphine C57BL/6 10–12 19–23 65,30 24 h: 19%
2d: 28%
3d: 30%
4d: 33%
7d: 33%
6, 12, 24 h
2, 3, 5, 7 days
2016 Young et al Metabolic scaling predicts post-hepatectomy liver regeneration after accounting for hepatocyte hypertrophy 8–12   68 0, 12, 24, 36, 48, 60, 72, 96 h
2016 Xie et al Quantification of Hepatic Vascular and Parenchymal Regeneration in Mice
2017 Will et al Longitudinal micro-computed tomography monitoring of progressive liver regeneration in a mouse model of partial hepatectomy Suture ligature of upper left posterior and left anterior lobe C57BL/6 N 11–12 18–26 42 1, 2, 3, 7 days
2018 Ozawa et al Evaluation of safety for hepatectomy in a novel mouse model with non-alcoholic steatohepatitis Single suture ligature of left and middle lobe Ether C57BL/6 J 6 70 0% 6, 12 h
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  1. A systematic search was conducted using Pubmed, gathering publications, which engaged with the mouse model of extended liver resection as a method itself. The results of this search were filtered according to their language, novelty, and relevancy. The Boolean search found 3741 articles on Pubmed, with 3130 publications remaining when filtered by language and the presence of a full text. In total, 21 of these publications examined the critical themes of the animal model described
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