Table 1 Sequences of primers used for RT-PCR
From: LncRNA NEAT1 promotes nucleus pulposus cell matrix degradation through regulating Nrf2/ARE axis
Gene | Forward primers (5′-3′) | Reverse primers (5′-3′) |
|---|---|---|
GAPDH | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG |
Col II | TGCTGCCTTTTCTGTTCCTT | AAGGTGCTGGGTAGGGAAGT |
Aggrecan | ACATCTCAGCAGCATCATCACC | CATCACCACGCAGTCCTCAC |
MMP-3 | ATGCCCACTTTGATGATGATGAAC | CCACGCCTGAAGGAAGAGATG |
MMP-13 | CGGTTCCGCCTGTCTCAAG | CGCCAAAAGTGCCTGTCTT |
lncRNA NEAT1 | TGGCTAGCTCAGGGCTTCAG | TCTCCTTGCCAAGCTTCCTTC |