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Fig. 3 | European Journal of Medical Research

Fig. 3

From: Thrombin-activated platelet-rich plasma enhances osteogenic differentiation of human periodontal ligament stem cells by activating SIRT1-mediated autophagy

Fig. 3

Effects of activated PRP on the autophagy of hPDLSCs. hPDLSCs were treated with or without thrombin-activated PRP. A Western blotting analyses demonstrated the expression of SIRT1, LC3-I, LC3-II and Beclin-1. GAPDH was used as an internal control and the quantitative analysis. B Autophagic flux was determined by treatment of Bafilomycin A1 in control cells or cells treated with 20% PRP and protein levels of LC3 II/I and Beclin-1 were measured. *P < 0.05, **P < 0.01

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