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Table 1 Calculation of suitable concentration of antigen and sera

From: Serological responses to a soluble recombinant circumsporozoite protein-VK210 of Plasmodium vivax (rPvCSP-VK210) among Iranian malaria patients

Positive sera

Negative sera

Sera dilution

1/20

1/40

1/80

1/160

1/20

1/40

1/80

1/160

Dilution Ag (μg/ml)

1

2

3

4

5

6

7

8

0.5

0.915

0.953

0.633

0.566

0.392

0.201

0.206

0.207

0.25

0.860

0.740

0.627

0.582

0.233

0.252

0.251

0.238

0.125

0.822

0.654

0.519

0.441

0.266

0.298

0.320

0.297

0.062

0.867

0.701

0.745

0.691

0.267

0.263

0.280

0.284

  1. The best titration result for the antigen concentration was 0.5 μg/ml, for the human sera it was a 1:40 dilution and for the IgG conjugate it was a 1:1000. The numbers 1–8 represent the wells of ELISA plates
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European Journal of Medical Research

ISSN: 2047-783X

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