Fig. 2

Knockdown of HIP1R inhibits the proliferation of thyroid cancer cells. A Protein level of HIP1R in NTHY-ORI 3–1, SW579, TPC-1 and 8505C cells examined by Western blot. GAPDH was the internal reference. B Grey value of HIP1R normalized to that of GAPDH calculated by Image J. C The mRNA level of HIP1R in NTHY-ORI 3–1, SW579, TPC-1 and 8505C cells examined by qRT-PCR. β-actin was the internal control. D Cell viability at 12, 24, 36, 48 and 60 h in SW579 and TPC-1 cells transfected with siCtrl or siHIP1R, respectively, examined by CCK-8 assay