Fig. 4

HIP1R induces endocytosis of PTEN in thyroid cancer cells. A The interaction between HIP1R and PTEN in SW579 cells examined by co-IP. B–D: SW579 cells were transfected with siCtrl or siHIP1R for 48 h, respectively. B Protein levels of membrane-binding PTEN and intracellular PTEN in SW579 cells. GAPDH was the internal control. C Fluorescence staining of PTEN in SW579 cells. Scale bar: 10 μm. D Subcellular distribution ratio of intracellular PTEN and membrane-binding PTEN in 100 SW579 cells