Fig. 5

Overexpression of FOSL1 reversed the circCRIM1 shRNA-induced inhibition of NPC cell invasion, proliferation and migration. SUNE-1 or CNE2 cells were treated with the control, sh-NC, sh-circCRIM1, sh-NC + FOSL1 or sh-circCRIM1 + FOSL1. Then, A the circCRIM1, miR-34c-p and FOSL1 levels in NPC cells were determined by RT–qPCR. B The FOSL1 levels in NPC cells were determined by Western blot. GAPDH was used for normalization. C NPC cell viability was assessed by the MTT assay. D NPC cell invasion and migration were assessed by the transwell assay. ^**P < 0.01. All data are expressed as the mean ± standard deviation (SD)