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Fig. 3 | European Journal of Medical Research

Fig. 3

From: Knockdown of circ_0003928 ameliorates high glucose-induced dysfunction of human tubular epithelial cells through the miR-506-3p/HDAC4 pathway in diabetic nephropathy

Fig. 3

The effect of circ_0003928 depletion on HG-induced HK-2 cell damage. AL HK-2 cells were treated with NG, HG, HG + si-NC and HG + si-circ_0003928, respectively, and circ_0003928 expression was analyzed by qRT-PCR (A), cell viability by a CCK-8 assay (B), cell proliferation by an EdU assay (C), ROS level by a cellular ROS assay kit (D), MDA production by a lipid peroxidation MDA assay kit (E), SOD activity by a Superoxide dismutase activity assay kit (F), caspase 3 activity by a caspase 3 activity assay (G), cell apoptotic rate by flow cytometry analysis (H), and the protein expression of Bax, cleaved caspase 3 and Bcl 2 by Western blotting analysis (IL). **P < 0.01 and ***P < 0.001

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