Fig. 6

Circ_0003928 modulated HDAC4 expression through miR-506-3p. A The schematic illustration showed the complementary sites of miR-506-3p with HDAC4 3′UTR. B and C HDAC4 expression was detected by qRT-PCR and Western blotting in the HK-2 cells induced by Mannitol, NG or HG. D The dual-luciferase reporter assay was used to determine the interaction of miR-506-3p with HDAC4 3′UTR. E The effect of miR-506-3p depletion on HDAC4 protein expression was determined by Western blotting analysis. F The effects between circ_0003928 knockdown and miR-506-3p inhibitors on HDAC4 protein expression were analyzed by Western blotting analysis. **P < 0.01 and ***P < 0.001