Skip to main content
Fig. 7 | European Journal of Medical Research

Fig. 7

From: Inhibitors of IFN gene stimulators (STING) improve intestinal ischemia–reperfusion-induced acute lung injury by activating AMPK signaling

Fig. 7

Compound C reverses the anti-pyroptotic and anti-apoptotic effects of C-176 against ALI in mice exposed to IR. A Representative photomicrographs of GSDMD-positive cells and C TUNEL-positive cells in the lung. B The number of GSDMD-positive cells and D the percentage of TUNEL-positive cells in the lung caused by the indicated stimuli 3 h after IR. Scale bar = 50 μm. E Representative Western blot of phospho-AMPK, total AMPK, cleaved caspase-1 and cleaved caspase-3 in the lung caused by the indicated stimuli. F The ratio between the optical density value of phospho-AMPK and total AMPK in the lung, as evaluated by Western blot. G The ratio between the optical density value of cleaved caspase-1 and GAPDH in the lung, as evaluated by Western blot; H the ratio between the optical density value of cleaved caspase-3 and GAPDH in the lung, as evaluated by Western blot. I The expression of IFNβ mRNA in the lung caused by the indicated stimuli. Data are presented as the mean ± SD (n = 6). Sham, IR, C-176, and vehicle are described above. ****P < 0.0001, ***P < 0.001, **P < 0.01

Back to article page