Fig. 2

Silence of BCAR3inhibited cell migration and invasion in CRC. A Western blot was used to measure the protein levels of the BCAR3 and GAPDH protein in SW1116 cells transfected with BCAR3 siRNA expression plasmid. GAPDH was used as the loading control. B MTT assay was performed on SW1116 cells transfected with BCAR3 siRNA expression plasmid. C, D Colony formation assay was performed on SW1116 cells transfected with BCAR3 siRNA expression plasmid. E, F Cell migration was analyzed using scratch wound assay (bar, 200 μm); **p < 0.01. (G, H) Cell invasion was analyzed using transwell assay (bar, 100 μm); **p < 0.01