Fig. 6

AMPK α2 promoted renal urate crystal deposition in a mouse model of chronic HN. a Representative images of eosin staining and compensation polarization microscopy of the entire anhydrous ethanol-treated kidneys. b Quantitative analysis of urate crystal deposition area in the kidneys. c Uric acid concentration per unit of renal tissue protein concentration. d–f Western blot analysis of AMPK α2 and MRP4 expression in the kidneys. g Genotyping of AMPK α2 knockout mice. M: DNA marker; 1, 2, wild-type homozygote, only DNA band near the 200 bp; 3, 4, heterozygote, DNA band of near both 200 bp and 600 bp; 5, 6, knockout homozygote, only DNA band near 600 bp. Each bar represents the mean ± SEM. Versus CON, *P < 0.05 and ***P < 0.001; versus WT, ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001