Fig. 2
Serotonin regulates the repetitive behavior of Drosophila. Trh01 mutant male flies show more grooming numbers (A) but spent nearly the same time per grooming episode (B) as well as total time spent grooming (C) when compared with Canton-S control flies during a 5-min observation period (n = 10–15 flies for each genotype). D Grooming numbers of Trh knockdown male flies during a 5-min observation period. E After feeding 2 mg/mL 5-HTP for 3 days, Trh01 mutant male flies displayed decreased grooming numbers. Error bars are shown as the mean ± SEM. For all data, *p < 0.05, **p < 0.01, ***p < 0.001