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Fig. 4 | European Journal of Medical Research

Fig. 4

From: Autism-like behaviors regulated by the serotonin receptor 5-HT2B in the dorsal fan-shaped body neurons of Drosophila melanogaster

Fig. 4

5-HT2B knockout flies showed further social distance and more severe repetitive behavior. A Schematic of two sgRNAs separately targeting the upstream region and first exon of the 5-HT2B locus. Orange boxes represent exons. Approximately 233 base pairs would be theoretically deleted when Cas9 is expressed in Drosophila germ cells. B, C Both 5-HT2B knockout lines showed further distance to their nearest neighbors in males (B) or females (C). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. D No climbing defect was observed in 5-HT2BKO_5−1 male or female flies or 5-HT2BKO_46−2 males, while 5-HT2BKO_46−2 females showed a slight defect in climbing. E, F More grooming numbers were detected in these 2 5-HT2B knockout lines in both sexes. Error bars are shown as the mean ± SEM (D, E and F)

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