Fig. 5

5-HT2B receptor in dFB neurons regulates the social interaction and repetitive behavior of Drosophila. A Expression pattern of 5-HT2B-Gal4, dFB-Gal4 (dorsal fan-shaped body neurons), per-Gal4 (period neurons), pdf-Gal4 (large ventral lateral clock neurons), or 83b-Gal4 (odorant receptor neurons) in the brain is visualized by UAS-mCD8: GFP (green). Areas located by different neurons are labeled: dFB (orange), per (dark green), ILNvs (blue), or83b (light green). B, C Quantification of the distance to the closest neighbor of targeted knockdown of 5-HT2B in different parts of brain neurons by expression of UAS-5-HT2B-RNAi under control of different Gal4. The distances between each fly of dorsal fan-shaped body-specific 5-HT2B knockdown (dFB-Gal4 > 5-HT2B-RNAi) flies were significantly longer than those of control flies. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. D, E Number of grooming episodes of different Gal4-specific 5-HT2B knockdown flies. Both males and females of the dFB-Gal4 > 5-HT2B-RNAi genotype showed more grooming numbers. F, G Climbing ability of 5-HT2B knockdown flies detected by negative geotaxis assay. Error bars are shown as the mean ± SEM (D-G)