Fig. 5

miR-155-5p modulated SOCS5/JAK2/STAT3 to step up MLE-12 cell injury mediated by LPS. MLE-12 cells were transfected along with miR-155-5p mimics and then with miR-155-5p mimics plus SOCS5 overexpression plasmid. LPS (1 μg/mL) was taken to treat the cells for 24 h. A qRT-PCR confirmed miR-155-5p expression in MLE-12 cells. B, C BrdU and CCK8 examined MLE-12 cell proliferation and viability. D Flow cytometry tracked MLE-12 apoptosis. E Western blot determined the profiles of apoptosis-concerned proteins in MLE-12 cells. F, G ELISA checked the levels of TNF-α, IL-6, IL-1β, and IL-10 in MLE-12 cells. H Western blot figured out the profile of the SOCS5/JAK2/STAT3 pathway in MLE-12 cells. *P < 0.05, **P < 0.01, ***P < 0.001 (vs. the control group); &P < 0.05, &&P < 0.01, &&& P < 0.001 (vs. the LPS group); NS P > 0.05, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 (vs. the LPS + miR-155-5p group). N = 3