Fig. 1

The role of hsa_circ_0023404 knockdown in endometrial cancer cells. The total RNA of HEEC and Ishikawa、RL95-2、 KLE endometrial cancer cell lines were collected and subjected to RT-qPCR. RT-qPCR analysis shows the level of hsa_circ_0023404 (A)、miR-217 (B) and MARK1 mRNA (C) in HEEC and Ishikawa、RL95-2、 KLE endometrial cancer cell lines, respectively. Ishikawa cell was transfected with control si-NC and si-circ_0023404 #1–3 for 48 h and subjected to RT-qPCR analysis. The level of hsa_circ_0023404 was knockdown by si-circ_0023404(si-circ) #1–3 in Ishikawa cell analyzed by RT-qPCR (D). The level of hsa_circ_0023404 (E) and miR-217 (F) was analyzed by RT-qPCR after si-circ #3 transfection. Cell proliferation was evaluated by CCK-8 assay (G) and colony formation assay (H, I) in si-NC or si-circ_0023404 transfected Ishikawa cells. The migration and invasion capacity of Ishikawa cell cells was determined by Transwell migration and invasion assay after transfected with si-NC or si-circ_0023404 (J). The statistics of Transwell migration (K) and invasion (L) assay for Ishikawa cells transfected with si-NC or si-circ_0023404