Fig. 4

Local increase in intrahepatic Tregs after splenectomy. Splenectomy induced excessive numbers of local Tregs in liver tissue (A) Cryo-preserved liver sections were immunohistochemically stained for CD4 (blue), CD8 (green) and Foxp3 (red). B The staining was quantified, as were the ratio of Tregs among CD4⁺ cells, (C) and the ratios of CD4⁺ and CD8⁺ T cells in splenectomized (n = 5) and non-splenectomized mice (n = 5)