Fig. 1

Flow chart of 16 s deoxyribonucleic acid assay for intestinal flora. PCR polymerase chain reaction, DNA deoxyribonucleic acid. In the raw data obtained from sequencing, there is a certain amount of interference data. To obtain high-quality sequencing data to improve the accuracy of the subsequent bioinformatics analysis, first, the original data needed to be spliced; then, they were quality controlled and filtered to obtain valid data