Fig. 3

TRMT6 promotes cell proliferation by regulating the PI3K/AKT signaling pathway. HepG2 cells transfected with shTRMT6 were treated with PI3/AKT signaling pathway activator IGF1, and grouped into control group, shTRMT6 group and shTRMT6 + IGF1 group. A CCK-8 assay was used to detect the cell activity. **P < 0.01 vs control or shTRMT6 group. B EDU cell proliferation detection was used to detect the number of EDU positive cells. C, D Cell cycle distribution was detected by flow cytometry. *P < 0.05, **P < 0.01 vs control or shTRMT6 group. E Protein expressions of p-PI3K, p-AKT and p-mTOR in HepG2 cells were detected by Western blot