Skip to main content

Table 1 Differences between differential ultracentrifugation (DUC) and ultrafiltration (UF)

From: Emerging role of mesenchymal stem/stromal cells (MSCs) and MSCs-derived exosomes in bone- and joint-associated musculoskeletal disorders: a new frontier

 

UF

DUC

Isolation process

Based on particle size and molecular weight cut-off (MWCO) of the utilized filter membrane

Physical features of exosomes, the exerted centrifugal force, and the viscosity of the solvent

Purity

Low

High

Specificity

Low

Intermediate

Time of isolation process

High

High

Complexity

Low

Intermediate

Sample volume

High

Intermediate

Cost

Intermediate

Low

Functionality of exosomes

Intermediate

Intermediate

Exosomes yield

High

Intermediate

Scalability

High

Intermediate

Efficiency

Intermediate

Intermediate

  1. Purity: The aptitude of isolating exosomes with minimum contamination Specificity: The capability to isolate exosomes from non-exosomal content Sample volume: The essential quantity of starting material Efficiency: Sample processing with substantial quality Complexity: The requirement for training beforehand the procedure Scalability: The ability to separate exosomes from large sample volumes without overly enhancing time, cost, or personnel required