Fig. 2

Validation of differentially binding lectins among different groups by lectin blot. a and d Lectin blot of SBA for serum IgG was performed in 16 patients with TAK (8 inactive and 8 active), 16 DCs, and 16 HCs. The signal intensity of IgG measured by Western blot was used as an internal control. The fluorescence intensity of the lectin blot band of the same healthy subject was used as a reference to correct for differences among membranes. The expression of SBA signal was normalized to IgG. The SBA/IgG of the TAK group was significantly higher compared with that of DCs and HCs. b and e Lectin blot of ConA for serum IgG between patients with inactive (n = 24) and active (n = 24) TAK. The ConA/IgG showed no significant difference between patients with inactive and active TAK (p = 0.0685). c and f Lectin blot of MNA-M for serum IgG between patients with inactive (n = 24) and active (n = 24) TAK. The MNA-M/IgG in the active TAK group was significantly lower than that in the inactive TAK group (p = 0.0319). SBA, Glycine max lectin; ConA, Concanavalin A lectin; MNA-M, Morniga M lectin; R reference