Fig. 3

B7-H3-CAR-T cells have a specific elimination effect on B7-H3-positive acute myeloid leukaemia (AML) cell lines. A Flow cytometry analysis showed that the positive rate of B7-H3-CAR-T cells was approximately 51.84%. B Western bolt analysis of B7-H3-CAR expression in T cells. Proteins from T (lane 1) and B7-H3-CAR T (lane 2) cells were extracted and separated via sodium dodecyl sulphate–polyacrylamide gel electrophoresis. After incubation with CD3Z chain-specific monoclonal antibody and horse radish peroxidase-goat anti-mouse IgG, protein bands were detected by chemiluminescence. C After 48 h of incubation an E:T ratio of 1:1, the proportion of apoptotic target cells was detected by flow cytometry. ****P < 0.0001, n = 3. In the figure, +++, ++ and + represent the high, medium and low expression of B7-H3 in cells, respectively. D. Supernatant obtained following co-incubation, interleukin-2 (IL-2), Tumour Necrosis Factor (TNF), interferon gamma (INF-γ), and granzyme B cytokine secretion. ****P < 0.0001, ***P < 0.001, **P < 0.01 by Student’s t test. C and D represent one of three independent parallel experiments, each reaction was carried out in triplicate, n = 3 wells/group