Fig. 5
B7-H3-CAR-T cells can eliminate tumour cells from acute myeloid leukaemia (AML) xenograft mice. A Schematic diagram of the AML transplantation model in B-NSG mice. Three female NSG mice (6–8 weeks old) in each group (PBS, T cell, and B7-H3-CAR-T cell groups) were injected with 5 × 105 Luciferase-GFP-HEL cells via the tail vein, and then injected with 2 × 107 effector cells in an equal volume in the tail vein on the second day. An equal volume of PBS was injected into the mice in the PBS group. B In vivo imaging of mice showing disease progression. n = 8 mice/group. C After erythrocytes were lysed from mouse peripheral blood and bone marrow cells, cells were incubated with CD45 antibodies, and the proportion of tumour cells was detected by flow cytometry. ****P < 0.0001, *P < 0.05, n = 3 mice/group. D Immunohistochemistry of B7-H3 in the spleen and ovarian tissues of the mice showed that, compared with the B7-H3-CAR-T cell group, there was significant tumour cell infiltration in the PBS and T cell groups at a magnification of ×400 and ×100, n = 3 mice/group