Fig. 2
The effect of complement C3 on renal pathology during post-injury fibrosis. a Hematoxylin and eosin (HE) stain and Masson’s trichrome of kidney tissue. Scale bar of glomerular = 25 μm; Scale bar of renal tubular = 50 μm. b Immunohistochemical detection of the expression of Synaptopodin, Nephrin, CD2AP, TRPC6 (Scale bar = 25 μm) in mice of each group. c Compare the levels of podocyte functional protein (IOD) of kidney tissues in each group, n = 10. d Western blot gel electrophoresis film exposure image e Quantitative analysis of the result, expressed as the ratio of target protein/β-actin (OD), n = 10. f Morphological changes of glomerular podocytes in each group of mice (TEM, × 3500): kidney cortical tissue was cut to 1mm3 tissue for electron microscopy, n = 10. ** P < 0.01vs WT-sham or C3−/−-sham, ## P < 0.01vs WT-AKI-CKD