Figure 2
Digestion of restricted plasmid DNA (pEGFP-N1, generated by restriction with EcoRI and NotI) by exhaled airway condensate or DNase I (0.05 U/μg DNA). M: Molecular marker; P1: Restriction fragments of plasmid DNA and DNase 0.05 U/μg DNA; P2: Restriction fragments of plasmid DNA and DNase 0.05 U/μg in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; AK 1 first lane: Restriction fragments of plasmid DNA and 130 μl airway condensate 1; second lane: Restriction fragments of plasmid DNA and 130 μl airway condensate 1 in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; AK 2 first lane: Restriction fragments of plasmid DNA and 130 μl airway condensate 2; second lane: Restriction fragments of plasmid DNA and 130 μl airway condensate 2 in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; AK3-CF first lane: Restriction fragments of plasmid DNA and 130 μl airway condensate of CF patient L.L.; second lane: Restriction fragments of plasmid DNA and 130 μl airway condensate of CF patient L.L. in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; AK4-CF first lane: Restriction fragments of plasmid DNA and 130 μl of airway condensate of CF patient H.H.; second lane: Restriction fragments of plasmid DNA and 130 μl of airway condensate of CF patient H.H. in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2; N1: plasmid DNA (pEGFP-N1) generated by restriction with EcoRI and NotI; N2: plasmid DNA (pEGFP-N1) generated by restriction with EcoRI and NotI in 1 mM EDTA, 1 mM EGTA, 10 mM MgCl2.