Figure 10c

Overlay of the densitogram curves before and after trypsin-incubation of the white muscle APFII. Figure 10c shows the enzyme incubation of 200 μg white muscle APFII with 65 μg trypsin (see Material). The batches, incubated with trypsin had a final concentration of 20 mM Tris/HCl, 20 mM β-mercaptoethanol, pH 8.0. After incubation at 37°C for a given time (30 min) in a waterbath, the reaction were irreversible inhibited by an final concentration of 10% w/v TCA (final concentration 5% TCA, 20 min, 0°C).