Western blotting analysis of Col III, α-SMA, TGF-β1 and TGF-βRII. Liver tissues from rats in different groups were lysed in the lysis buffer. Samples were centrifuged to pellet cell debris, mixed with SDS-PAGE sample buffer. After boiled for 5~10 min, 40 μg of protein was electrophoresed on a 10% SDS-polyacrylamide gel. The protein was transferred to PVDF membrane. The blots were incubated with the primary antibody against Col III, α-SMA, TGF-β1, TGF-βRII and β-actin (1: 400) at 4°C overnight respectively and subsequently with corresponding secondary antibody (1: 1000) at room temperature for 2 h. Protein bands were visualized with an ECL Western blotting detection system kit and signal was captured on X-ray film.