Figure 1
Construction of the randomly fragmented cDNA library in pTKO2 vector. A) Plasmids that contained randomly cDNA libraries under the control of pSV40. HygR, hygromycin; AmpR, ampicillin; EBNA-1, Epstein-Barr virus nuclear antigen type, coding sequence; OriP, Episomal origin of replication; splice/Poly(A)+, splice and polyadenylation signal derived from SV40. B) Schematic diagram of the functional screening for genes with pro-apoptotic function during CD95-mediated (CH11-triggered) apoptosis in tumour cells, using the original (one cycle selection)- and the modified (multi-cycle selection) TKO strategy.